New insights into the bacterial targets of antimicrobial blue light
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Microbiology Spectrum
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Antimicrobial blue light (aBL) offers efficacy and safety in treating infections.
However, the bacterial targets for aBL are still poorly understood and may be dependent
on bacterial species. Here, we investigated the biological targets of bacterial killing by aBL
(l = 410 nm) on three pathogens: Staphylococcus aureus, Escherichia coli, and Pseudomonas
aeruginosa. Initially, we evaluated the killing kinetics of bacteria exposed to aBL and used
this information to calculate the lethal doses (LD) responsible for killing 90 and 99.9% of
bacteria. We also quantified endogenous porphyrins and assessed their spatial distribution.
We then quantified and suppressed reactive oxygen species (ROS) production in bacteria to
investigate their role in bacterial killing by aBL. We also assessed aBL-induced DNA damage,
protein carbonylation, lipid peroxidation, and membrane permeability in bacteria. Our data
showed that P. aeruginosa was more susceptible to aBL (LD99.9 = 54.7 J/cm2) relative to
S. aureus (LD99.9 = 158.9 J/cm2) and E. coli (LD99.9 = 195 J/cm2). P. aeruginosa exhibited the
highest concentration of endogenous porphyrins and level of ROS production relative to
the other species. However, unlike other species, DNA degradation was not observed in
P. aeruginosa. Sublethal doses of blue light (,LD90) could damage the cell membrane in
Gram-negative species but not in S. aureus. In all bacteria, oxidative damage to bacterial
DNA (except P. aeruginosa), proteins, and lipids occurred after high aBL exposures (.LD99.9).
We conclude that the primary targets of aBL depend on the species, which are probably
driven by variable antioxidant and DNA-repair mechanisms.
Como referenciar
ANJOS, CAROLINA dos; LEANSE, LEON G.; RIBEIRO, MARTHA S.; SELLERA, FÁBIO P.; DROPA, MILENA; ARANA-CHAVEZ, VICTOR E.; LINCOPAN, NILTON; BAPTISTA, MAURÍCIO S.; POGLIANI, FABIO C.; DAI, TIANHONG; SABINO, CAETANO P. New insights into the bacterial targets of antimicrobial blue light. Microbiology Spectrum, v. 11, n. 2, p. 1-13, 2023. DOI: 10.1128/spectrum.02833-22. Disponível em: http://repositorio.ipen.br/handle/123456789/34104. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.