Antimicrobial photodynamic therapy challenges microbial drug-resistance
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2019
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SÃO PAULO SCHOOL OF ADVANCED SCIENCE ON MODERN TOPICS IN BIOPHOTONICS
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Background: The rising challenge of microbial resistance to chemotherapy demands immediate implementation of
global policies and therapeutic alternatives. Antimicrobial photodynamic therapy (APDT) combines the
administration of a photosensitizer (PS) compound with low-intensity monochromatic light to induce
photochemical reactions that yield high amounts of reactive oxygen species (ROS). Since some PS molecular
frameworks can be selectively incorporated by pathogens and ROS react with virtually all biomolecules, APDT
offers a powerful strategy to challenge microbial resistance of local infections. Methods: In this study we assayed
the APDT efficacy, using methylene blue (MB) as PS and red light provided by LED, against planktonic
suspensions of high-risk representative fungal and bacterial species. The species tested include A. baumannii
(OXA-23 and 143), E. aerogenes (NDM-1), E. faecalis (VAN-B), E. faecium (VAN-A), E. coli (MCR-1, CTX-M8
and 15), K. pneumoniae (KPC-2, IMP-1, OXA-48), S. aureus (MRSA, VISA), P. aeruginosa (VIM-1, SPM-1, GES-
5), C. albicans and C. neoformans. For all species, we tested standard control strains compared to azole-resistant
yeast, or bacteria resistant to nearly all commercially available antimicrobials, in attempt to observe any crossresistance
in between APDT and standard chemotherapy. Results: More than 5log10 reduction was observed
within less than a minute of illumination for non-capsulated bacteria and within less than 5 minutes for yeast and
capsulated bacteria. Regardless of resistance phenotype MB-APDT presented species-specific dose-response
kinetics suggesting that similar therapeutic protocols may bring successful outcomes in clinical practice.
Conclusions: Our study proposes that MB-APDT can efficiently inactivate a broad-spectrum of drug-resistant
microorganisms and impair drug-resistance genes selection and dissemination.
Como referenciar
SABINO, C.P.; RIBEIRO, M.S.; BAPTISTA, M.S.; LINCOPAN, N. Antimicrobial photodynamic therapy challenges microbial drug-resistance. In: SÃO PAULO SCHOOL OF ADVANCED SCIENCE ON MODERN TOPICS IN BIOPHOTONICS, March 20-29, 2019, São Carlos, SP. Abstract... p. 61-61. Disponível em: http://repositorio.ipen.br/handle/123456789/30196. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.