From micro to nano Raman
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2022
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ENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7.
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The environmental accumulation of nanoplastics formed by material of anthropic origin has raised doubts about their
safety, especially to the human body. While microplastics are accidentally consumed, nanoplastics (NPs) are even more
concerning as they are much more likely to be absorbed by human body cells. It is known that plastics smaller than 200
nm can penetrate cell membranes and cross the blood-brain barrier. Studies have shown that polystyrene (PS) NPs
from the environment carry a high load of toxins capable of compromising human brain cells. Very little is still known
about what effects, cytotoxic or not, these plastics have on different organs. Understanding the property-function
relationship of nanoparticles in various fields of application involves determining their physicochemical properties, which
is still a formidable challenge to date. Our project focuses on the development of a methodology for the detection of
micro- and NPs using micro-Raman, TERS (Tip Enhanced Raman Spectroscopy), collinear Raman and AFM, nuclear
techniques, as well as a methodology for in vitro evaluation of the toxic effects of these materials through biochemical
assays of cytotoxicity and genotoxicity. The project contemplates the determination of the adsorption capacity of metallic
ions by NPs and the absorption of micro- and nanoplastics in cell cultures with radioactive tracers, the determination of
microplastics in tissues from necropsies of marine animals and gamma spectroscopy of the cellular incorporation of NPs
labeled with radioactive isotopes.
In figure 1 we show examples of (a) the detection of microplastics from the Santos basin and comparison to Raman
reference database (KnowItAll®), (b) detection of NPs in mouse fibroblast cells and (c) detection of very small particles
(50 nm) which can be achieved by co-localized techniques of AFM + Raman using special, narrow (50 m), femtosecond
laser written gratings on quartz.
Como referenciar
WETTER, NIKLAUS; FREITAS, ANDERSON Z.; BERECZKI, ALLAN; PASCOAL, DIEGO R.C.; DIPOLD, JESSICA; PARRA, DUCLERC; ROSSI, WAGNER; CABRAL, FERNANDA V.; RIBEIRO, MARTHA S. From micro to nano Raman: new developments in the detection of micro and nanoplastics of marine origin from the Amazon basin to Santos. In: ENCONTRO BRASILEIRO DE ESPECTROSCOPIA RAMAN, 7., 5-8 de dezembro, 2022, São Pedro, SP. Resumo... Disponível em: http://repositorio.ipen.br/handle/123456789/33685. Acesso em: 21 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.