Cytotoxicity model using human cardiomyocytes derived from pluripotent cells (iPSCs) for cardiotoxicity safety assessment
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2017
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WORLD CONGRESS ALTERNATIVES AND ANIMAL USE IN THE LIFE SCIENCES, 10th
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Resumo
In contemporary drug development, preclinical and clinical evaluation, cardiac safety concerns arise from a
variety of drug-tissue interactions, including direct myocyte toxicity. In this study, in vitro cytotoxic studies were
conducted on GLP conditions in human cardiomyocytes derived from pluripotent cells (iPSCs). Before testing, cell
population purity, above 90%, were confirmed by troponin 1 antibody biomarker. iPSCs cells were incubated with
DMSO and Doxorubicin for 48 hours in a 96-well plates. Three different dyes – MTS, MTT and NR – were used to
evaluate cell viability. The results showed that the iPSCs derived cardiomyocyte model was sensitive to predictive
moderate and severe drug-induced cardiotoxicity. According to these results, the in vitro cytotoxicity model using
iPSCs derived cardiomyocytes can be applied in the safety assessment of novel drug candidates as well as to
identify compounds that may cause cardiotoxicity.
Como referenciar
DAMIANI, RENATA; VALGODE, FLAVIA; PASSOS, PRISCILA; VALADARES, MARCOS; BIAGI, DIOGO; HIGA, OLGA; MEDEIROS, FABIANA. Cytotoxicity model using human cardiomyocytes derived from pluripotent cells (iPSCs) for cardiotoxicity safety assessment. In: WORLD CONGRESS ALTERNATIVES AND ANIMAL USE IN THE LIFE SCIENCES, 10th, August 20-24, 2017, Seattle, Washington, USA. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/28437. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.