Cytotoxicity model using human cardiomyocytes derived from pluripotent cells (iPSCs) for cardiotoxicity safety assessment

Abstract

In contemporary drug development, preclinical and clinical evaluation, cardiac safety concerns arise from a variety of drug-tissue interactions, including direct myocyte toxicity. In this study, in vitro cytotoxic studies were conducted on GLP conditions in human cardiomyocytes derived from pluripotent cells (iPSCs). Before testing, cell population purity, above 90%, were confirmed by troponin 1 antibody biomarker. iPSCs cells were incubated with DMSO and Doxorubicin for 48 hours in a 96-well plates. Three different dyes – MTS, MTT and NR – were used to evaluate cell viability. The results showed that the iPSCs derived cardiomyocyte model was sensitive to predictive moderate and severe drug-induced cardiotoxicity. According to these results, the in vitro cytotoxicity model using iPSCs derived cardiomyocytes can be applied in the safety assessment of novel drug candidates as well as to identify compounds that may cause cardiotoxicity.