Silver nanoparticles reduced by tannic acid and sodium citrate
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective: Green nanotechnology aims to reduce hazardous chemical waste in the
environment through sustainable development. Phytochemicals are proposed to minimize environmental
impacts and produce safe biological applications. Silver nanoparticles (AgNPs) have effective antimicrobial
properties against Gram-positive and Gram-negative bacterias, fungi and viruses, being promising to reduce
the microbial load. Antimicrobial systems based on AgNPs shows promise in combating bacteria.
Methodology: Confirmation of the formation of silver nanoparticles was evaluated by UV-Vis
spectrophotometry. The hydrodynamic size and polydispersion index were evaluated by dynamic light
scattering. The zeta potential was used to assess stability through surface charge. The obtained morphology
and average size were evaluated by transmission electronic microscopy. The cytotoxicity assay was performed
to assess cellular viability of silver nanoparticles in HUVEC cells. The antimicrobial activity was analyzed by
minimum inhibitory concentration through microdilution in broth and later the inoculum was performed in
plates.
Results and discussion: Characterization by spectrophotometry of AT_AgNPs and CT_AgNPs showed
absorption bands at 430 nm. Hydrodynamic size analyzes revealed diameters of 57.87-97.45 nm, with
polydispersion indices (PdI) ranging between 0.289 and 0.392. The zeta potential was determined between -
4.41 and -10.3 mV. Transmission electron microscopy (TEM) images revealed spherical morphology with sizes
between 20-50 nm. AgNPs have been tested as a treatment against hospital microorganisms with risk
classification level 2, including Gram-negative (Acinetobacter baumannii, Escherichia coli, Klebsiella
pneumoniae and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria. The
Minimum Inhibitory Concentration (MIC) was determined to evaluate the lowest concentration that inhibits
the growth of microorganisms. The MIC values obtained with the AT_AgNPs were: 4.18 ug/mL (AB14 and
EC23), 8.36 ug/mL (EC26 and KP43), 16.72 ug/mL (PA17), 66.9 ug/mL (PA3) and 133.8 ug/mL (SA). For
CT_AgNPs, they were: 51.8 ug/mL (AB14, EC26 and PA17), without inhibition in EC23, KP43, PA3 and SA
strains. Synergism was evaluated by mixing suspensions of the two nanoparticles (ATCT_AgNPs), resulting in
MIC values of 1.48 ug/mL (PA3 and SA), 2.96 ug/mL (EC23, KP43 and PA17) and 5.93 ug/mL (AB14 and EC26).
Conclusions: The results suggest that AT_AgNPs and the synergistic combination with CT_AgNPs have
potential as effective antimicrobial agents against nosocomial bacteria. These AgNPs may represent a
promising alternative for the development of new therapeutic strategies to combat bacterial infections.
However, further studies are needed to investigate its activity in other contexts and its safety in clinical use.
Como referenciar
RODRIGUES, ADRIANA S.; BATISTA, JORGE G.S.; MINARINI, LUCIENE A.R.; LOPES, PATRICIA; LUGAO, ADEMAR B. Silver nanoparticles reduced by tannic acid and sodium citrate: a synergic approach with antimicrobial properties. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 240. Disponível em: https://repositorio.ipen.br/handle/123456789/47927. Acesso em: 03 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.