Post-column derivatization high-performance liquid chromatography-ultraviolet-ultraviolet (HPLC-UV) method for the detection of Kryptofix 2.2.2 content in Positron Emission Tomography (PET) tracers
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Nuclear Medicine and Biology
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A post-column derivatization high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed to determine Kryptofix 2.2.2 (K2.2.2) in PET tracers. This method utilizes the specific complexation between K2.2.2 and Pb2+, forming a stable 1:1 host-guest complex. The complexation produces a bathochromic shift in UV absorption maximum (from 210 nm to 254 nm), enabling sensitive detection. This approach addresses the limitations of poor performance and narrow applicability associated with direct single-method detection of K2.2.2. The chromatographic conditions were optimized (column flow rate: 0.7 mL·min−1; derivatization reagent flow rate: 0.7 mL·min−1; Pb2+ concentration: 50 μg·mL−1) and the method was validated. The results exhibited excellent linearity (1–100 μg·mL−1, r2 > 0.999), high reproducibility (relative standard deviation (RSD) < 5%), and a low detection limit (0.5 μg·mL−1). The method successfully quantified K2.2.2 in [18F]FDG, [18F]AV45 and [18F]DPA714, achieving spiked recoveries of 89%–105%. Additionally, the method remained unaffected by interference from common sample components such as sodium chloride and sodium ascorbate. This method provides a novel and efficient quality control tool for the simultaneous analysis of radiochemical purity and K2.2.2 content in diverse PET tracers.
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XU, LEI; DONG, ZHENHAO; ZHANG, HONG; GOMES, ANTONIO A.; ROSSI, WAGNER de; WANG, JING; NARIO, ARIAN P.; HE, QINGGANG. Post-column derivatization high-performance liquid chromatography-ultraviolet-ultraviolet (HPLC-UV) method for the detection of Kryptofix 2.2.2 content in Positron Emission Tomography (PET) tracers. Nuclear Medicine and Biology, v. 154-155, p. 1-8, 2026. DOI: 10.1016/j.nucmedbio.2026.109610. Disponível em: https://repositorio.ipen.br/handle/123456789/50199. Acesso em: 13 Oct 2026.
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