A novel strategy based on Zn(II) porphyrins and silver nanoparticles to photoinactivate candida albicans
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International Journal of Nanomedicine
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Background: Photodynamic inactivation (PDI) is an attractive alternative to treat Candida albicans infections, especially considering
the spread of resistant strains. The combination of the photophysical advantages of Zn(II) porphyrins (ZnPs) and the plasmonic effect
of silver nanoparticles (AgNPs) has the potential to further improve PDI. Here, we propose the novel association of polyvinylpyrrolidone (PVP) coated AgNPs with the cationic ZnPs Zn(II) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin or Zn(II) meso-tetrakis
(N-n-hexylpyridinium-2-yl)porphyrin to photoinactivate C. albicans.
Methods: AgNPs stabilized with PVP were chosen to allow for (i) overlap between the NP extinction and absorption spectra of ZnPs
and (ii) favor AgNPs-ZnPs interaction; prerequisites for exploring the plasmonic effect. Optical and zeta potential (ζ) characterizations
were performed, and reactive oxygen species (ROS) generation was also evaluated. Yeasts were incubated with individual ZnPs or
their respective AgNPs-ZnPs systems, at various ZnP concentrations and two proportions of AgNPs, then irradiated with a blue LED.
Interactions between yeasts and the systems (ZnP alone or AgNPs-ZnPs) were evaluated by fluorescence microscopy.
Results: Subtle spectroscopic changes were observed for ZnPs after association with AgNPs, and the ζ analyses confirmed AgNPsZnPs interaction. PDI using ZnP-hexyl (0.8 µM) and ZnP-ethyl (5.0 µM) promoted a 3 and 2 log10 reduction of yeasts, respectively.
On the other hand, AgNPs-ZnP-hexyl (0.2 µM) and AgNPs-ZnP-ethyl (0.6 µM) systems led to complete fungal eradication under the
same PDI parameters and lower porphyrin concentrations. Increased ROS levels and enhanced interaction of yeasts with AgNPs-ZnPs
were observed, when compared with ZnPs alone.
Conclusion: We applied a facile synthesis of AgNPs which boosted ZnP efficiency. We hypothesize that the plasmonic effect
combined with the greater interaction between cells and AgNPs-ZnPs systems resulted in an efficient and improved fungal inactivation. This study provides insight into the application of AgNPs in PDI and helps diversify our antifungal arsenal, encouraging further
developments toward inactivation of resistant Candida spp.
Como referenciar
RAPOSO, BRUNO L.; SOUZA, SUEDEN O.; SANTANA, GLEYCIANE S.; LIMA, MAX T.A.; SARMENTO-NETO, JOSE F.; REBOUCAS, JULIO S.; PEREIRA, GORETI; SANTOS, BEATE S.; CABRAL FILHO, PAULO e; RIBEIRO, MARTHA S.; FONTES, ADRIANA. A novel strategy based on Zn(II) porphyrins and silver nanoparticles to photoinactivate candida albicans. International Journal of Nanomedicine, v. 18, p. 3007-3020, 2023. DOI: 10.2147/IJN.S404422. Disponível em: http://repositorio.ipen.br/handle/123456789/34245. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.